City Research Online

Analysis of capillary-like structures formed by endothelial cells in a novel organotypic assay developed from heart tissue

Bowyer, S., Kanthou, C. and Reyes-Aldasoro, C. C. (2014). Analysis of capillary-like structures formed by endothelial cells in a novel organotypic assay developed from heart tissue. In: Reyes-Aldasoro, C. C. and Slabaugh, G.G. (Eds.), Medical Image Understanding and Analysis 2014. (pp. 226-231). London: British Machine Vision Association. ISBN 1-901725-51-0

Abstract

In this work, we present an algorithm to perform automatic segmentation, feature extraction and dosage classification of images derived from a novel organotypic angiogenesis assay developed to assess the effects of ionising radiation in the mouse heart. The images presented very different conditions of illumination and conditions of density and shapes of cells. The algorithm consists of a pipeline of several steps, which were validated against hand-segmented images. The algorithm provided satisfactory results, as all images were correctly dose-classified. The cells exposed to the lowest radiation dose were observed to have the greatest relative feature variability.

Publication Type: Book Section
Subjects: R Medicine > R Medicine (General)
T Technology > TA Engineering (General). Civil engineering (General)
Departments: School of Mathematics, Computer Science & Engineering > Engineering
School of Mathematics, Computer Science & Engineering > Engineering > Electrical & Electronic Engineering
Related URLs:
URI: http://openaccess.city.ac.uk/id/eprint/3953
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