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Measuring cellular migration with image processing

Reyes-Aldasoro, C. C., Biram, D., Tozer, G. M. and Kanthou, C. (2008). Measuring cellular migration with image processing. Electronics Letters, 44(13), pp. 791-793.

Abstract

An image-processing algorithm for the analysis of migration of vascular endothelial cells in culture is presented. The algorithm correctly detected the cellular regions on either side of an artificial ‘wound’ made by dragging a sterile pipette tip across the monolayer of cells (scratch wound assay). Frequency filtering and mathematical morphology were used to approximate the boundaries of cellular regions. This allowed the measurement of the distance between the regions, and therefore the migration rates, regardless of the orientation of the wound and even in cases where the cells were sparse and not tightly packed.

Publication Type: Article
Additional Information: This paper is a postprint of a paper submitted to and accepted for publication in Electronic Letters and is subject to Institution of Engineering and Technology Copyright. The copy of record is available at IET Digital Library
Subjects: R Medicine > RC Internal medicine
Departments: School of Mathematics, Computer Science & Engineering > Engineering
School of Mathematics, Computer Science & Engineering > Engineering > Electrical & Electronic Engineering
URI: http://openaccess.city.ac.uk/id/eprint/4311
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