City Research Online

Abstract

The aim of this work is to register serial in-vivo confocal microscopy images of zebrafish to enable accurate cell tracking on corresponding fluorescence images. The following problem arises during acquisition; the zebrafish tail may undergoe a series of movement and non-linear deformations, which if not corrected, adds to the motion of leukocytes being tracked. This makes it difficult to accurately assess their motion. We developed a correlation based, local affine image matching method, which is well suited to the textured DIC images of the anatomy of the zebrafish and enables accurate and efficient tracking of image regions over successive frames. Experimental results of the serial registration and tracking demonstrate its accuracy in estimating local affine motions in zebrafish sequences.

Publication Type:	Conference or Workshop Item (Paper)
Additional Information:	© 2012 IEEE. Personal use of this material is permitted. Permission from IEEE must be obtained for all other uses, in any current or future media, including reprinting/republishing this material for advertising or promotional purposes, creating new collective works, for resale or redistribution to servers or lists, or reuse of any copyrighted component of this work in other works.
Subjects:	T Technology > TA Engineering (General). Civil engineering (General)
Departments:	School of Science & Technology > Engineering School of Science & Technology > Computer Science > giCentre

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