City Research Online

An on-line chromatic and scale-space microvasculature-tracing analysis for transmitted light optical images

Reyes-Aldasoro, C. C., Björndahl, M. A., Akerman, S. , Ibrahim, J. & Tozer, G. M. (2011). An on-line chromatic and scale-space microvasculature-tracing analysis for transmitted light optical images. In: http://www.biomedical-image-analysis.co.uk/. Medical Image Understanding and Analysis, 14-07-2011 - 15-07-2011, London UK.

Abstract

Limited contrast in optical images from intravital microscopy is problematic for analysing tumour vascular morphology. Moreover, in some cases, changes in vasculature are visible to a human observer but are not easy to quantify. In this paper two quantitative on-line algorithms are presented: scale-space vessel tracing and chromatic decomposition for tumour vasculature from in-vivo transmitted light optical images. The algorithms were tested on intravital window chamber images of the vasculature from SW1222 human colorectal carcinomas, which were treated with a vascular disrupting agent combretastatin-A-4-phosphate (CA-4-P) or saline. The results confirmed the well-known effects of CA-4-P on the constriction of vessels. Furthermore, changes in the chromaticity suggest a deoxygenation of the blood with a recovery to initial levels in CA-4-P-treated tumours relative to the controls. The algorithms can be freely applied to any vascular image through the CAIMAN (CAncer IMage ANalysis: http://www.caiman.org.uk).

Publication Type: Conference or Workshop Item (Paper)
Additional Information: Copyright 2011. The copyright of this document resides with its authors. It may be distributed unchanged freely in print or electronic forms.
Publisher Keywords: vessel tracing, chromatic analysis, tumour vasculature
Subjects: R Medicine > RE Ophthalmology
T Technology > TK Electrical engineering. Electronics Nuclear engineering
Departments: School of Science & Technology > Engineering
School of Science & Technology > Computer Science > giCentre
[thumbnail of MIUA2011R.pdf]
Preview
PDF - Published Version
Download (360kB) | Preview

Export

Add to AnyAdd to TwitterAdd to FacebookAdd to LinkedinAdd to PinterestAdd to Email

Downloads

Downloads per month over past year

View more statistics

Actions (login required)

Admin Login Admin Login