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Automatic segmentation of focal adhesions from mouse embryonic fibroblasts

Reyes-Aldasoro, C. C., Barri, M. & Hafezparast, M. (2015). Automatic segmentation of focal adhesions from mouse embryonic fibroblasts. In: 2015 IEEE 12th International Symposium on Biomedical Imaging (ISBI). (pp. 548-551). IEEE. doi: 10.1109/ISBI.2015.7163932


This work describes an automatic algorithm for the segmentation and quantification of focal adhesions from mouse embryonic fibroblasts. The main challenges solved by this algorithm are: the variability of the intensity of the focal adhesions, the detection of an outer ring, which distinguishes the cell periphery responsible for the cell migration, and the quantification of the characteristics of the focal adhesions. The algorithm detects maximal regions through gradients and uses a region-growing algorithm limited by intensity-based edges. The outer ring is calculated based on the average radial intensity from an extended centroid of the cell. Finally, traditional morphological characteristics are obtained to distinguish between two groups of cells. Two of the measurements employed showed statistical difference between two groups of cells.

Publication Type: Book Section
Additional Information: © 2015 IEEE. Personal use of this material is permitted. Permission from IEEE must be obtained for all other uses, in any current or future media, including reprinting/republishing this material for advertising or promotional purposes, creating new collective works, for resale or redistribution to servers or lists, or reuse of any copyrighted component of this work in other works.
Publisher Keywords: cell segmentation, mouse embryonic fibroblasts, MEF, focal adhesions
Subjects: R Medicine > R Medicine (General)
T Technology > TA Engineering (General). Civil engineering (General)
Departments: School of Science & Technology > Engineering
School of Science & Technology > Computer Science > giCentre
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