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Comparative Study of Contact Repulsion in Control and Mutant Macrophages Using a Novel Interaction Detection

Solís-Lemus, J. A., Sánchez-Sánchez, B. J., Marcotti, S., Burki, M., Stramer, B. and Reyes-Aldasoro, C. C. ORCID: 0000-0002-9466-2018 (2020). Comparative Study of Contact Repulsion in Control and Mutant Macrophages Using a Novel Interaction Detection. Journal of Imaging, 6(5), 36.. doi: 10.3390/jimaging6050036

Abstract

In this paper, a novel method for interaction detection is presented to compare the contact dynamics of macrophages in the Drosophila embryo. The study is carried out by a framework called macrosight, which analyses the movement and interaction of migrating macrophages. The framework incorporates a segmentation and tracking algorithm into analysing the motion characteristics of cells after contact. In this particular study, the interactions between cells is characterised in the case of control embryos and Shot mutants, a candidate protein that is hypothesised to regulate contact dynamics between migrating cells. Statistical significance between control and mutant cells was found when comparing the direction of motion after contact in specific conditions. Such discoveries provide insights for future developments in combining biological experiments with computational analysis.

Publication Type: Article
Additional Information: ⃝c 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Subjects: Q Science > QA Mathematics > QA75 Electronic computers. Computer science
Q Science > QH Natural history > QH301 Biology
Departments: School of Mathematics, Computer Science & Engineering > Computer Science
Date Deposited: 12 Jun 2020 12:49
URI: https://openaccess.city.ac.uk/id/eprint/24274
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