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Prophase-Specific Perinuclear Actin Coordinates Centrosome Separation and Positioning to Ensure Accurate Chromosome Segregation

Stiff, T., Echegaray-Iturra, F. R., Pink, H. J., Herbert, A., Reyes-Aldasoro, C. C. ORCID: 0000-0002-9466-2018 and Hochegger, H. (2020). Prophase-Specific Perinuclear Actin Coordinates Centrosome Separation and Positioning to Ensure Accurate Chromosome Segregation. Cell Reports, 31(8), 107681.. doi: 10.1016/j.celrep.2020.107681

Abstract

Centrosome separation in late G2/ early prophase requires precise spatial coordination that is determined by a balance of forces promoting and antagonizing separation. The major effector of centrosome separation is the kinesin Eg5. However, the identity and regulation of Eg5-antagonizing forces is less well characterized. By manipulating candidate components, we find that centrosome separation is reversible and that separated centrosomes congress toward a central position underneath the flat nucleus. This positioning mechanism requires microtubule polymerization, as well as actin polymerization. We identify perinuclear actin structures that form in late G2/early prophase and interact with microtubules emanating from the centrosomes. Disrupting these structures by breaking the interactions of the linker of nucleoskeleton and cytoskeleton (LINC) complex with perinuclear actin filaments abrogates this centrosome positioning mechanism and causes an increase in subsequent chromosome segregation errors. Our results demonstrate how geometrical cues from the cell nucleus coordinate the orientation of the emanating spindle poles before nuclear envelope breakdown.

Publication Type: Article
Additional Information: Copyright © 2020 The Author(s). This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
Publisher Keywords: centrosome separation, Eg5, LINC complex, FHOD1, perinuclear actin, microtubules, centrosome positioning, G2/M transition, mitotic entry, centrosome tracking
Subjects: Q Science > QA Mathematics > QA75 Electronic computers. Computer science
Q Science > QH Natural history > QH301 Biology
Departments: School of Mathematics, Computer Science & Engineering > Computer Science > giCentre
Date Deposited: 25 Jun 2020 09:55
URI: https://openaccess.city.ac.uk/id/eprint/24394
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