City Research Online

Investigations into the Effects of pH on Quantitative Measurements of Lactate in Biological Media Using ATR-FTIR Spectroscopy

Baishya, N., Mamouei, M. H., Budidha, K. ORCID: 0000-0002-6329-8399, Qassem, M. ORCID: 0000-0003-0730-3189, Vadgama, P. and Kyriacou, P. A. ORCID: 0000-0002-2868-485X (2020). Investigations into the Effects of pH on Quantitative Measurements of Lactate in Biological Media Using ATR-FTIR Spectroscopy. Molecules, 25(16), 3695.. doi: 10.3390/molecules25163695


Quantification of lactate/lactic acid in critical care environments is essential as lactate serves as an important biochemical marker for the adequacy of the haemodynamic circulation in shock and of cell respiration at the onset of sepsis/septic shock. Hence, in this study, ATR-FTIR was explored as a potential tool for lactate measurement, as the current techniques depend on sample preparation and fails to provide rapid response. Moreover, the effects of pH on PBS samples (7.4, 7, 6.5 and 6) and change in solution conditions (PBS to whole blood) on spectral features were also investigated. A total 189 spectra from five sets of lactate containing media were obtained. Results suggests that lactate could be measured with more than 90% accuracy in the wavenumber range of 1500-600 cm-1. The findings of this study further suggest that there exist no effects of change in pH or media, when estimating lactate concentration changes in this range of the Mid-IR spectral region.

Publication Type: Article
Additional Information: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Publisher Keywords: lactate; lactic acid; infrared spectroscopy; ATR; sepsis
Subjects: R Medicine > R Medicine (General)
T Technology > TK Electrical engineering. Electronics Nuclear engineering
Departments: School of Mathematics, Computer Science & Engineering > Engineering > Electrical & Electronic Engineering
Date Deposited: 03 Sep 2020 12:58
Text - Published Version
Available under License Creative Commons: Attribution International Public License 4.0.

Download (291kB) | Preview



Downloads per month over past year

View more statistics

Actions (login required)

Admin Login Admin Login