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Novel luminescent compounds for immunoassay

Blincko, S. (1989). Novel luminescent compounds for immunoassay. (Unpublished Doctoral thesis, City, University of London)


The thesis describes the synthesis of ruthenium bipyridyl and phenanthro1ine compounds that are luminescent. Several of the compounds were suitably derivatised for protein conjugation i.e. [Please see inside of thesis for formulas]

The excitation and emission spectra were recorded for the above compounds and their luminescent lifetimes determined. Some of the above compounds were conjugated to antibody samples and their luminescent characteristics recorded. It was found that the complex-antibody conjugates had similar properties to their parent complexes possessing a large Stoke's Shift (about 130-140 nm) and a long luminescent lifetime (125-1200 ns). Hence these compounds may be of use as protein labels in time resolved immunoassays.

The thesis also describes the synthesis of some luminescent chromium and aluminium compounds i.e. [Please see inside of thesis for formulas]

Their luminescent characteristics were investigated. The preparation of several oxine derivatives was also attempted with varying success.
The thesis ends with an epilogue discussing science as a God-given activity.

Publication Type: Thesis (Doctoral)
Subjects: Q Science > QD Chemistry
Departments: School of Science & Technology
School of Science & Technology > School of Science & Technology Doctoral Theses
Doctoral Theses
[thumbnail of Blincko thesis 1989 PDF-A.pdf]
Text - Accepted Version
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